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Selected genes regulated by BMP4 in micromass cultures 2-fold p < 0.05.

Selected genes regulated by BMP4 in micromass cultures 2-fold p

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Selected genes regulated by TGF-β in micromass cultures 2-fold, p < 0.05.

Selected genes regulated by TGF-β in micromass cultures 2-fold, p

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Selected vertebrae enriched genes that are up-regulated in Tgfbr2 -deleted...

Selected vertebrae enriched genes that are up-regulated in Tgfbr2 -deleted IVD.

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Selected IVD enriched genes that are down-regulated in the IVD by deletion...

Selected IVD enriched genes that are down-regulated in the IVD by deletion of Tgfbr2 .

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IVD enriched, verified genes.

IVD enriched, verified genes.

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Scatter plot analysis

Scatter plot analysis

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Verification of regulation of selected genes by TGF-β using RT-PCR

Verification of regulation of selected genes by TGF-β using RT-PCR

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Sclerotome culture

Sclerotome culture

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Hierarchical clustering analysis

Hierarchical clustering analysis

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Verification of localization by in situ hybridization

Verification of localization by in situ hybridization

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Laser Microdissection

Laser Microdissection

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Body Weights at Study Initiation and Completion a

Body Weights at Study Initiation and Completion a

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Bone mineral density (BMD) at the femoral midshaft showed no significant...

Bone mineral density (BMD) at the femoral midshaft showed no significant difference between control and Mg-deficient mice

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Bone mineral content (BMC) of the femoral metaphysis at baseline (time 0)...

Bone mineral content (BMC) of the femoral metaphysis at baseline (time 0) and 2, 4 and 6 weeks of study

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The results of the comparison by the Analysis of Variance (ANOVA) (p = 0,05)...

The results of the comparison by the Analysis of Variance (ANOVA) (p = 0,05) of the mean levels of expression of BMP-2 and the levels of BMP-4 in the groups of examined bones. S – significant...

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Evaluation of significant differences between mean levels of BMP-4...

Evaluation of significant differences between mean levels of BMP-4 expression in all examined bones. S – significant difference (p 0,05)

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Evaluation of significant differences between mean levels of BMP-2...

Evaluation of significant differences between mean levels of BMP-2 expression in all examined bones. S – significant difference (p 0,05)

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The number and codes of examined skeletal bone samples

The number and codes of examined skeletal bone samples

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Primer sequences and sizes of amplicons generated by Real-time RT-PCR

Primer sequences and sizes of amplicons generated by Real-time RT-PCR

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Electrophoretic analysis of real-time PCR products

Electrophoretic analysis of real-time PCR products

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Melting curves of real-time PCR products

Melting curves of real-time PCR products

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Expression of BMPs in different bone biopsies derived from 6 patients...

Expression of BMPs in different bone biopsies derived from 6 patients quantified by real-time PCR

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Comparison of BMP-4 expression levels in separate bone groups

Comparison of BMP-4 expression levels in separate bone groups

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Comparison of BMP-2 expression levels in separate bone groups

Comparison of BMP-2 expression levels in separate bone groups

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(a) Schematic representation of the rat osteocalcin (OC) gene promoter...

(a) Schematic representation of the rat osteocalcin (OC) gene promoter transcribing at basal levels. The circle in the middle represents a positioned nucleosome flanked by a distal and proximal DNase...

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Osteoclast differentiation, activation, and regulation. Stages of maturation...

Osteoclast differentiation, activation, and regulation. Stages of maturation illustrated with regulatory factors driving the differentiation stages and final adhesions and activity of the...

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Stages and markers of osteoblast growth and differentiation. (a) Bone...

Stages and markers of osteoblast growth and differentiation. (a) Bone surface in vivo shows organization of osteoblast lineage cells as they maturate in relation to the mineralizing bone. (b) Primary...

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